Figure 2
From: Quantifying extracellular matrix turnover in human lung scaffold cultures
Culture of fibroblasts in decellularized lung scaffolds. Representative images from scaffolds at four different time points; day 6, 9, 12 and 25, are shown, including an outer edge of the scaffold at the top of each image in A–D. (A) HE staining of repopulated scaffolds at four time points. Cell nuclei are seen dispersed within the scaffold and lining the edge of the scaffold. (B) Ki-67 staining (red) for proliferating cells with nuclear DAPI counterstain (blue), arrows: Ki-67 positive cells. (C) TUNEL staining indicating few apoptotic cells (yellow) at each time point, arrows: TUNEL positive cells. (D) Staining for cytoskeletal protein Vimentin (yellow) showing the location of cell bodies. The basement membrane protein Collagen type IV (red) visualizes the scaffold, cell nuclei stained with DAPI (blue). Scale bars: 50 µm. (E) The percentage of Ki-67 positive cells quantified in six entire tissue sections from two or three individual 3D scaffolds for each time point (error bars show SD). (F) Cell density as quantified by the summed histone intensities at all time points, error bars show SD.
