Figure 2
From: Vitamin C promotes apoptosis in breast cancer cells by increasing TRAIL expression
Vitamin C treatment elevates 5hmC content in breast cancer cells. (a) The dot-blot shows that treatment with 10, 100, and 500 μM vitamin C for 24 hours increases the global content of 5hmC. (b) The semi-quantitation of the dot-blot shows that 10 μM vitamin C treatment for 24 hours increases the global content of 5hmC, supplementation to 100 μM further increases 5hmC levels, but further supplementation to 500 μM does not increase the levels further. (c) The dot-blot shows that treatment with 100 μM vitamin C increases the global content of 5hmC. (d) The semi-quantitation of the dot blot shows that 1 day of treatment with 100 μM vitamin C treatment increases the global content of 5hmC, and that supplementation for longer periods largely maintains the 5hmC level (* indicates P < 0.05).
