Figure 4
From: Vitamin C promotes apoptosis in breast cancer cells by increasing TRAIL expression
Vitamin C increases the expression of TRAIL. (a) The mRNA levels of TRAIL, measured by qRT-PCR, are increased in MDA-MB-231 cells treated for 3 days with 100 µM vitamin C. (b) The levels of TRAIL protein are increased, measured by Western blot, in MDA-MB-231 cells treated for 7 days with 100 µM vitamin C. (c) The secretion of TRAIL protein is increased, measured by ELISA test, in the culture media of MDA-MB-231 cells treated for 3 days with 100 µM vitamin C. (* indicates P < 0.05). (d) MDA-MD-231 cells transfected with scramble RNAi show an increase in TRAIL mRNA when treated with Vitamin C, but when transfected with TETs RNAi, vitamin C treatment does not elevate the TRAIL mRNA levels.
