Figure 1 | Scientific Reports

Figure 1

From: Enhanced labeling density and whole-cell 3D dSTORM imaging by repetitive labeling of target proteins

Figure 1

The principle of SERI and its technical implementation. (a) Fluorophore-labeled trisNTA binds to His6/His10-tagged proteins and enables dSTORM super-resolution imaging. trisNTA can be eluted using a buffer containing imidazole. This procedure of labeling, imaging and elution can be repeated many times, with each repetition providing a super-resolution image. (b) Schematic depiction of the restaining setup. Two circulating pumps with plastic tubing allow for solution exchange during washing steps.

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