Figure 1
Heterochromatin landscape in the different states of mouse pluripotency (A) Single plan images of the 3 cell types immunolabeled with antibodies against H3K9 tri-methylation (H3k9me3) and H3K27 tri-methylation (H3K27me3). DNA is counterstained with DAPI. Scale bars represent 5µm. Encircled nuclei correspond to each of the 4 categories sketched in B. (B) Schematic drawings of the 4 categories of nuclei observed within the 2i-ESC population and their proportion in the 3 cell types. The number of cells analysed is indicated in the first column. (C) Boxplots showing the number of H3K9me3 and H3K27me3 clusters in positive nuclei. Pvalues were calculated using Student t-test; n.s. = not significant. (D) Western-blot analysis of H3K27me3 and H3K9me3 with H3 total as loading control in the 3 cell types. (E) ChIP-qPCR analysis of H3K9me3 and H3K27me3 at major satellites and beta-Actin promoter (to measure background level). Stars indicate statistically significant data and error bars are s.e.m (n = 4, Mann-Whitney U test, P value ≤ 5%).
