Figure 3

Methylation profile at major satellites. (A) Southern-blot analysis of gDNA digested with HpyCH4IV revealed with probe for major satellites. Linescan quantification for each lane: MEF (black), DnmtTKO (pink), 2i-ESC (red), serum-ESC (blue) and EpiSC (green). (B) Western-blots for DNMT3A, DNMT3B and H3.