Figure 5
Inhibition of H3K27me3 in 2i-ESCs. (A) Western-blot analysis of EZH2, H3K9me3 and H3K27me3 related to total H3 in 2i-ESC treated with DMSO (Control) or EPZ (EZH2 inhibition). (B) Immunostaining images (single-plan) for H3K9me3 and H3K27me3 with DAPI DNA counterstaining in 2i-ESC treated with DMSO or EPZ. Percentages of cells presenting H3K9me3 clusters are indicated. Scale bars represent 5 µm. (C) Boxplots showing the number of H3K9me3 clusters in 2i-ESC treated with DMSO or EPZ. p-values were calculated using Student t-test; n.s. = not significant. (D) Relative expression (CNRQ) of major satellites transcripts by qRT-PCR analysis normalized to Sdha and Pbgd housekeeping genes in 2i-ESC treated with DMSO or EPZ. Each point is an independent biological replicate. Statistically significant data are indicated by an asterisk (P < 5%, Mann-Whitney U test for paired data).
