Figure 7
Organization at PCH and major satellite transcript level in DnmtTKO cells. (A) Immunostaining images (single-plan) for H3K9me3 and H3K27me3 with DAPI DNA counterstaining in DnmtTKO condition. Encircled nuclei are representative examples of categories 1, 2 and 3. Scale bars represent 5µm. (B) Representative nuclei of the categories as defined in Fig. 1B and percentages of each in the 2i-ESC and cEpiSC populations. (C) Box-plots showing the number of clusters of H3K27me3 in positive cells in WT and mutant ESC and cEpiSC. Pvalues (student tests) are shown. (D) Relative expression (CNRQ) of major satellites transcripts by qRT-PCR analysis normalized to Sdha and Pbgd housekeeping genes in Wild-type and DnmtTKO condition.
