Figure 7
Effect of MLO-A5 conditioned medium, collagen, and hydroxyapatite on expression of Snail and p-Akt. (A) Comparison of mRNA levels of EMT genes in TMD and BMD tumor cells, and the TMD response to MLO-A5 and MLO-Y4 conditioned media. (B) Multi-dimensional scaling analysis of TMD response to MLO-A5 and MLO-Y4 conditioned media. (C) Expression changes of Snail and p-Akt in response to MLO-A5 conditioned media, collagen, and hydroxyapatite administration. Full-length Western blot images are presented in Supplementary Fig. S3. (D & E) Effects of collagen administration on the wound healing of TMD cells. (F) Effect of collagenase-treated conditioned medium on TMD tumor spheroids. (G) Effect of collagenase-treated conditioned medium on Snail expression. (H–I) Effect of col1a1 siRNA on TMD tumor spheroids and Snail expression.
