Figure 5
From: Directed -in vitro- evolution of Precambrian and extant Rubiscos
Expression of RubRr, ancestral Rubisco and their mutant offspring in E. coli. (a) Rubisco content as a percentage of the total soluble E. coli protein (TSP, %) determined by 14C-CABP binding and confirmed by (b) SDS-PAGE. Lane 1, RubRr; Lane 2, clone 11; Lane 3, clone 27; Lane 4, clone 25; Lane 5, clone 9; Lane 6, MRPro; Lane 7, clone B2; Lane 8, clone B3; Lane 9, clone B9; Lane 10, MW protein standard. The Rubisco RbcL subunit corresponds to the prominent 50 kDa band. Refer to Fig. 2a for the origins and mutations of each clone.
