Table 1 Biochemical characterization of extant and ancestral evolved Rubiscos.
From: Directed -in vitro- evolution of Precambrian and extant Rubiscos
Rubisco | In vitro Evolution strategy | Tm (°C) | [Rubisco] (% tsp1) | SC/O (mol.mol−1) | KC (μM) | kcatC (s-1) | kcatC/KC (mM.s-1) | KmRuBP (μM) |
|---|---|---|---|---|---|---|---|---|
RubRr | Modern parent | 70.7 | 28.1 ± 2.6 | 12.3 ± 0.2 | 105.2 ± 4.6 | 13.1 ± 1.1 | 125.2 ± 16 | 4.6 ± 1.5 |
Clone 9 | Genetic drift | 73.2 | 23.6 ± 1.3 | 11.6 ± 0.3 | 99.6 ± 1.1 | 12.9 ± 0.2 | 129.5 ± 2.9 | 5.4 ± 1.4 |
Clone 11 | Adaptive evolution | 74.5 | 31.0 ± 1.7 | 11.7 ± 0.2 | 318.7 ± 40.1 | 10.6 ± 0.9 | 33.6 ± 0.3 | 6.9 ± 0.9 |
Clone 25 | Adaptive evolution | 77.4 | 28.2 ± 1.2 | 12.7 ± 0.3 | 178.0 ± 4.1 | 8.1 ± 0.5 | 45.2 ± 1.9 | 45.9 ± 1.5 |
Clone 27 | Adaptive evolution | 74.2 | 34.6 ± 4.9 | 12.1 ± 0.2 | 111.5 ± 2.2 | 14.1 ± 1.1 | 126.5 ± 8.3 | 8.7 ± 0.8 |
MRPro | Ancestral parent | 69.0 | 45.0 ± 4.7 | 6.5 ± 0.2 | 97.7 ± 3.2 | 4.0 ± 0.2 | 40.7 ± 1.1 | 158.3 ± 16.2 |
Clone B2 | MORPHING | 75.0 | 44.9 ± 6.9 | 6.9 ± 0.1 | 198.2 ± 15.4 | 1.4 ± 0.1 | 7.1 ± 0.7 | 375.1 ± 42.9 |
Clone B3 | Adaptive evolution | 70.0 | 41.4 ± 2.6 | 6.2 ± 0.1 | 105.1 ± 6.3 | 3.8 ± 0.5 | 36.3 ± 2.9 | 164.4 ± 13.8 |
Clone B9 | Adaptive evolution | n.d. | 56.5 ± 3.3 | 7.7 ± 0.6 | 94.9 ± 2.7 | 3.5 ± 0.1 | 36.6 ± 2.5 | 206.7 ± 14.8 |
