Figure 3

Random forest (RF) analysis of the salivary microbiota in the three subject groups. RF is performed at the genus-level (A–C) and at the species level (D–F). The best RF model with the highest AUC is indicated by a red circle in the PSC-HC comparison (A), and a red triangle in the PSC-UC comparison (D). The taxa selected in the best RF model are shown in (B,E) for the PSC-HC comparison and in (C,F) for the PSC-UC comparison. The bars indicate the mean decrease Gini, and the colors indicate the fold-change, which were calculated by dividing the mean relative abundance (%) of each genus and species in the PSC by that in the HC group (B,E) and the UC group (C,F), respectively. PCA, principal component analysis.