Figure 4

Cell morphologies and alignment of HT-22 mouse hippocampal neuronal cells on the random PDO, aligned PDO, aligned PDO/Col, and aligned Lam-PDO/Col core-shell matrices. (A) Two-photon excitation fluorescence images of HT-22 mouse hippocampal neuronal cells on the random PDO, aligned PDO, aligned PDO/Col, and aligned Lam-PDO/Col core-shell matrices at 5 days of culture. (i-iv) Magnified images of the region enclosed by the dashed white square in (v–viii). The cell nuclei were counterstained with DAPI (blue), and the F-actins were stained with TRITC-labelled phalloidin (red). Yellow arrows indicate the direction of aligned fibers. All photographs shown in this figure are representative of six independent experiments with similar results. (B) Quantitative analysis of the cell alignment on the random PDO, aligned PDO, aligned PDO/Col, and aligned Lam-PDO/Col core-shell matrices. Note the distinctive pixel intensity created by the FFT output image containing aligned HT-22 cell information.