Figure 5
From: Aligned laminin core-polydioxanone/collagen shell fiber matrices effective for neuritogenesis
Immunofluorescence staining analysis of neurite outgrowth and HT-22 mouse hippocampal neuronal cell alignment on the random PDO, aligned PDO, aligned PDO/Col, and aligned Lam-PDO/Col core-shell matrices. (A) Two-photon excitation fluorescence images of neurite outgrowth. The HT-22 mouse hippocampal neuronal cells were cultured in growth media for 5 days, and further cultured in differentiation media for 7 days on the random PDO, aligned PDO, aligned PDO/Col, and aligned Lam-PDO/Col core-shell matrices. The cell nuclei were counterstained with DAPI (blue), and the neurofilaments were immunofluorescence stained with FITC-labelled antibody for the detection of neurofilaments (green). Yellow arrows indicate the direction of aligned fibers. All photographs shown in this figure are representative of six independent experiments with similar results. (B) Quantification of neurofilament-positive area and (C) average neurite outgrowth of HT-22 mouse hippocampal neuronal cell. An asterisk (*) denotes a significant difference compared with the other groups (p < 0.05). The data are presented as the average ± standard deviation of at least three independent experiments, each performed in duplicate on different cultures.
