Figure 3

Sirtuin 1 (SIRT1) mRNA expression and abundance. (A) fat (B) pancreas (C) dorsal hippocampus. Two-way ANOVA was used to assess the statistical significance of differences among groups and the therapeutic effect of resveratrol was evaluated by Mann-Whitney U test. Representative immunoblots and densitometric quantification of SIRT-1 are presented. Values are mean ± SEM. Represented full blots are presented in Supplementary Figure S2. n = 11–13 per group in SIRT1 mRNA expression and n = 6–8 in SIRT1 protein amount. There were one outlier in the pancreas and two outliers in the dorsal hippocampus SIRT1 protein abundance, respectively. *P < 0.05; **P < 0.01.