Figure 3

Transcriptome analysis of rudhira knockout. (a) Representation of differentially expressed genes in rudh^−/− by volcano plot. (b) Venn diagram showing number of genes (unique probe sets) dysregulated in embryo and yolk sac upon rudhira deletion. (c) Unsupervised hierarchical clustering of differentially expressed gene changes between control and rudh^−/− embryo (Emb) and yolk sac (YS). Green: low expression red: high expression. Genes with the most similar expression profiles clustered together with the shortest branches. Dendrogram illustrates their relationship. (d) Unsupervised hierarchical clustering of differentially expressed gene changes in rudh^−/− yolk sac compared to controls. Each row represents a gene, and column represents the tissue. (e) Histogram showing significantly enriched Gene Ontology (GO) and Pathways (p <  = 0.05) harboring differentially expressed genes in the embryo and yolk sac upon rudhira deletion. (f) Model depicting rudhira/BCAS3 gene regulatory pathway visualized with CytoscapeV8.0.