Figure 2 | Scientific Reports

Figure 2

From: Development of a characterised tool kit for the interrogation of NLRP3 inflammasome-dependent responses

Figure 2

Compounds targeting NLRP3 but not downstream caspase-1 inhibit nigericin-induced formation of ASC specks in THP1-ASC-Cerulean macrophages. THP1-ASC-Cerulean macrophages were seeded in 96 well plate format, differentiated with PMA (0.5 µM, 3 h), pre-treated with compound for 30 min (VX-765 (a), MCC950 (b) and NBC19 (c)) before being stimulated with nigericin (10 µM, 1 h), fixed and imaged on GE InCell2000. Specks can this way be visualized and counted. Compounds targeting the NLRP3 inflammasome assembly induced a dose-dependent reduction in the number of specks. Dose response curves (d) showing the number of formed specks and the cell death for each compound. Scale bar depicts 20 µm. Respective concentration of compound and DMSO controls are indicated in the images. Data represent 3 separate cultures.

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