Figure 1
From: A Multi-Parameter Analysis of Cellular Coordination of Major Transcriptome Regulation Mechanisms
Experimental strategy. Log-phase HCT116 cells were split up into three parts. One part was used to extract total mRNA for RNA-seq analysis to measure steady-state mRNA abundance (RA) (black texts and arrows). One part was used to perform nuclear run-on to generate bromo-UTP labeled nascent RNA for sequencing, that is, GRO-seq analysis to measure transcription rate (TR) (red texts and arrows). The last part was used to isolate and quantify polysome associated mRNA to measure translation activity (TA) (green texts and arrows).
