Figure 6 | Scientific Reports

Figure 6

From: Alternative SNP detection platforms, HRM and biosensors, for varietal identification in Vitis vinifera L. using F3H and LDOX genes

Figure 6

Representation of the sensor response with a SNP positioned in different locations in relation to the probe: (a) single strand DNA-Probe 35, Target SNP 5′ (one mismatch in the 5′ end of the target), and Target 15 (complementary); (b) single strand DNA-Probe 35, Target SNP center (one mismatch in the center of the target), and Target 15 (complementary); (c) single strand DNA-Probe 35, Target SNP 3′ (one mismatch in the 3′ end of the target), and Target 15 (complementary); (d) representation of the probe immobilization process on the fiber, with a detailed vision of the top of the fiber and the disposition of the probe, with a wider access in the 3′-end of the probe (orange arrow) favouring the beginning of the hybridization reaction with the samples that are present in the fiber’s surrounding environment.

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