Figure 1 | Scientific Reports

Figure 1

From: A Bowman-Birk type chymotrypsin inhibitor peptide from the amphibian, Hylarana erythraea

Figure 1

Identification and structural analysis of HECI. (A) RP-HPLC chromatogram of Hylarana erythraea skin secretion. The linear gradient of buffer B (0.05/19.95/80.0 (v/v/v) TFA/water/acetonitrile) is indicated in blue line. The arrow indicates the retention time of HECI, about 34% acetonitrile. (B) Chymotrypsin inhibition activity screening of HPLC fractions 98–102 min (○ 98 min, ● 99 min, ■ 100 min, □ 101 min, ▲ 102 min).

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