Figure 3
From: Plasmodium falciparum strains spontaneously switch invasion phenotype in suspension culture
Differential sensitivities of Static and Suspended parasites to enzyme treatment of erythrocytes. Invasion efficiencies of Static (ST) and Suspended (SP) into erythrocytes treated with neuraminidase (250 mU), chymotrypsin (1 mg/ml) and trypsin (1 mg/ml) were compared for P. falciparum Dd2, W2mef and 3D7 strains. Invasion rates were determined by flow cytometry as percentage of ring-infected erythrocytes after approximately 14 hours’ incubation of schizonts with target erythrocytes, and expressed as invasion efficiency relative to invasion of untreated erythrocytes. Data are presented as mean ± standard errors of three independent experiments performed in triplicates. *P-value < 0.05 at 95% CI; Student t-test.
