Figure 5 | Scientific Reports

Figure 5

From: Plasmodium falciparum strains spontaneously switch invasion phenotype in suspension culture

Figure 5

RH4 and PEBL are upregulated in suspended Dd2 and W2mef: Transcript levels of selected P. falciparum invasion related genes were determined by reverse transcription quantitative polymerase chain reaction analyses in Static (ST) and Suspended (SP) Dd2, W2mef and 3D7. The expression level of each gene was normalized to 60S ribosomal L18 protein as an endogenous control and then to AMA1 as a late stage marker. Relative fold changes were determined by normalizing the expression levels in Suspended parasites to Static parasites. (a) Represent transcript levels of all genes at week 12. The transcript level of RH4, PEBL, RH1 and PF3D7_1202400 at weeks (W) 2, 6 and 12 are shown in (b). All experiments were run in triplicates. Error bars represent standard errors. *Genes with ≥2-fold change in expression at week 12 in both Dd2 and W2mef.

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