Figure 7
From: Snf1/AMPK is involved in the mitotic spindle alignment in Saccharomyces cerevisiae
(a) wild type, kar9Δ and dyn1Δ strains expressing Snf1-3HA were grown in synthetic medium with 2% glucose and processed for western analysis with anti-pT172-AMPK or anti-HA antibodies. (b) Immunostaining for pT210-Snf1 using anti-pT172-AMPK antibodies and DAPI staining of DNA in wt and kar9Δ cells. bar: 5 µm. (c) Spindle morphology and (d) viability of kar9Δsnf1Δ[empty], kar9Δsnf1Δ[Snf1-wt] and kar9Δsnf1Δ[Snf1-G53R] in synthetic medium containing 2% glucose. *p < 0.05.
