Table 1 Steady-state kinetics of purified Luc proteins at pH 7.8.

From: A Firefly Luciferase Dual Color Bioluminescence Reporter Assay Using Two Substrates To Simultaneously Monitor Two Gene Expression Events

Enzyme

kcat (s−1, x10−3)

Km (µM)

kcat/Km (mM−1s−1)

LH2:BtLH2 (kcat/Km)

LH2

BtLH2

LH2

BtLH2

LH2

BtLH2

PpyWT

27 ± 3

2.8 ± 0.8

15 ± 2

6 ± 1

1.8

0.47

3.8

PLR1

12 ± 1

0.20 ± 0.03

37 ± 4

28 ± 3

0.32

0.007

45.7

PLG3

4.7 ± 0.3

2.3 ± 0.1

15 ± 3

5 ± 1

0.31

0.46

0.67

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