Figure 1
From: Carboxythiazole is a key microbial nutrient currency and critical component of thiamin biosynthesis
Ostreococcus tauri RCC745 grows on exogenous cHET (with HMP) in B1-deplete medium. Mean cell abundance data are for multiple days of the experiment (colored columns). (A) RCC745 grows when provided different concentrations of cHET (plus 1 nM HMP) or B1 (1 nM; as a positive control). The addition of cHET also facilitates use of low concentrations (pM) of HMP by RCC745 (Supplementary Fig. S2). (B) In contrast, a RCC745 ∆thiM mutant does not grow on supplied cHET (plus 1 nM HMP). Asterisks denote a significant difference (p < 0.05; n = 3; paired two-tailed t-test) relative to respective negative controls (−Con.).
