Figure 2
From: Carboxythiazole is a key microbial nutrient currency and critical component of thiamin biosynthesis
An E. coli ∆thiG mutant grows on B1-deplete M63 medium using exogenous cHET. All concentration values along the x-axes are in picomolar. (A) E. coli ∆thiG cells sustain growth using sub-picomolar concentrations of exogenous cHET or B1. (B) The E. coli ∆thiG mutant exhibited no notable growth upon supplied HET up to 105 pM, highly contrasting with responses to notably lower cHET additions. (C) Dramatically higher concentrations (≥106 pM) of exogenous HET are required to sustain growth of E. coli ∆thiG. Mean maximum yields for triplicate cultures are plotted along with their respective standard deviations. Asterisks denote significant differences (p < 0.05, n = 3; paired two-tailed t-test) versus the negative control (−Con.).
