Figure 4
From: Carboxythiazole is a key microbial nutrient currency and critical component of thiamin biosynthesis
An updated metabolic map including cHET in B1 salvage and de novo biosynthesis pathways. Orange arrows represent cHET utilization demonstrated here. Solid and dashed purple arrows represent usage and generation (via degradation) of HET reported previously1,2,4,30. Large black arrows denote core B1 biosynthesis processes. Cells acquire exogenous thiazole precursor via high (HA) or low affinity (LA) transport systems (with unknown sequence identity), or diffusion based on results shown here (Figs 1, 2, Supplementary Fig. S1) and in prior studies4,30. Shorthand compound names are in italics, while shorthand enzyme names are in bold. Thi80, TPK = thiamin pyrophosphate kinase; TMPsynthase = thiamin monophosphate synthase; P-tase = phosphatase; TMP = thiamin monophosphate; TDP = thiamin diphosphate; B1 = thiamin; −P = phosphate group; HMP = 4-amino-5-hydroxymethyl-2-methylpyrimidine; cHET = 5-(2-hydroxyethyl)-4-methyl-1,3-thiazole-2-carboxylic acid; HET = 4-methyl-5-thiazoleethanol.
