Figure 3
Effects of AGE-2 and AGE-3 on RAW 264.7 cell morphology and phagocytosis. Macrophages seeded at 8 × 105 cells/well were cultured for 4 h with AGE-2 or AGE-3 at 200 μg/ml. Transmission electron microscopy (TEM) indicated the morphological change of RAW264.7 cells treated by AGE-2 (a) or AGE-3 (b). TEM in combination with immunohistochemical analysis showed intracellular black particles, which reflect the incorporation of AGE-2 (c) or AGE-3 (d), both of which react with the anti-AGE Ab post-stained with a diaminobenzidine labelling procedure. All the micrographs in this figure were taken at the same magnification (see scale bar). The arrows in panel (c) or (d) show intracellular particles of AGE-2 or AGE-3 within a phagosome, respectively.
