Figure 3
Change in serum total IgE levels of mice sensitized with DNFB according to Protocol 5. (a) Protocol 5 involves three DNFB sensitizations at 14 day intervals. (b and c) Mice were painted with 100 µL of 0.15% DNFB in vehicle [acetone/olive oil (3:1)] (black circle of b, n = 3 and c, n = 7, pre and day 19, n = 3, day 26 and n = 4, day 27) or 100 µL of vehicle only (white circle of b, n = 3). (b) and (c) indicates different mice. Blood samples were collected from the legs at the indicated time points. The serum IgE concentrations were measured by sandwich ELISA. IgE values of the individual mice are presented as a line graph (three mice per group) (b) and as a box-plot with the median and IQR of 3–7 mice (c). *P < 0.05 and **P < 0.01, versus IgE values of pre-treatments. The x-axis is not drawn to scale. The arrows indicate DNFB painting. (d) The frequency of scratching behavior was observed 2 h after 2nd DNFB treatment. Data are presented as a box-plot with the median and IQR of six mice. **P < 0.01, versus vehicle-treated mice.
