Figure 4
From: Mechanical Tension Drives Elongational Growth of the Embryonic Gut
Quantitative morphometric changes in-ovo and in culture. (a) From a 2D photograph of the demesenterized midgut (step 1), a Voronoi tessellation algorithm extracts gut axis and contour (step 2), allowing to retrieve gut length, average diameter and volume (step 3: 3D reconstruction using the Olaf ImageJ plugin)43. (b) Physiological in-ovo volume, length and diameter changes at E9 and E10 relative to the state at E8 (n = 3 for each stage). (c) Volume, length and diameter changes induced by 48 h culture without tension (red squares, n = 27) or with tension (black diamonds, n = 95) as a function of the stress applied by the hanging mass (see Explanatory Note S3). Red dashed lines are trend guides. Each data point corresponds to a different gut. The data are the result of 21 independent experiments.
