Figure 5

Dry mass, cell size and cell number changes of cultured guts. Data of each figure is the result of at least 3 independent experiments, each point corresponds to a separate sample (gut). (a) Scheme of the method to measure dry mass (see Materials & Methods, Video S6, Figs S7, 8). (b) Dry mass variation of E8 guts cultured without (red squares, n = 6) or with (black squares, n = 10) applied tension. The stress applied was in the range 50–150 Pa. (c) Scheme of gut dissociation protocol and resulting brightfield, acridine orange/propidium iodide (AO/PI) and analyzed image of cells in counting chamber (see Materials & Methods). (d) Average measured cell size in E8 guts cultured without (n = 7) or with (n = 17) applied tension, and for native E8 (blue squares, n = 4) and E10 guts (green dots, n = 5). The stress applied was in the range 50–150 Pa. (e) Cell number change in E8 guts cultured without (n = 7) or with (n = 17) applied tension. *p < 0.05, Mann Whitney two-tailed test.