Figure 6
From: Mechanical Tension Drives Elongational Growth of the Embryonic Gut
Proliferation in cultured and native E8 guts. Phospho-histone S10 immunohistochemical staining of midgut section of E8 gut cultured for 48 H without tension (a), with tension (b) and for a native, uncultured E8 gut (c). Proliferating cells appear as GFP bright spots, overlaid over the brightfield image (gray). (d) Proliferation density (number of PH3 positive cells on a section divided by the section surface area) for n = 9 unweighed, n = 9 weighed (1 mg, stress in the range 70–140 Pa) and n = 4 uncultured E8 guts. The average proliferation density for each sample is obtained from n = 6–18 different slices. (e) Percentage of proliferating cells found in the epithelium. *p < 0.05, Mann Whitney two-tailed test.
