Figure 2
From: Anisomycin prevents OGD-induced necroptosis by regulating the E3 ligase CHIP
Anisomycin reduces necroptosis of N2a cells following OGD. 24 h prior to an OGD challenge, N2a cells were treated with or without 100 nM anisomycin or 10 μM SP600125. After 4 h OGD followed with 12 h recovery, tests were implemented (A) Cell counting kit was used to test the viability of each group. Significant differences bp < 0.05 vs. Control, ap < 0.05 vs. OGD (B) AnnexinV-FITC Apoptosis Detection Kit was used to test the necroptosis and apoptosis cells. (C) Propidium iodide (PI)-positive cells were analyzed after a 12 h recovery of OGD. Bars represent the mean ± SEM of four independent experiments. Significant differences *p < 0.05 vs. OGD, #p < 0.05 vs. OGD. (D) Apoptosis cells were analyzed after 4 h OGD and 12 h recovery. Bars represent the mean ± SEM of four independent experiments. Significant differences #p < 0.05 vs. OGD.
