Figure 7
From: Anisomycin prevents OGD-induced necroptosis by regulating the E3 ligase CHIP
CHIP is important for reducing RIPK3 levels in anisomycin treated OGD-challenged N2a cells. 24 h after transfection of N2a cells with PGPU6-GFP-shNC or PGPU6-GFP-siCHIP, cells were challenged with OGD for 4 h after treat with or without anisomycin for 24 h. (A) With 12 h of recovery, lysates of OGDR insulted N2a cells were analyzed by western blot to detect the quantitation of RIPK3. (B) The lysates from (A) were respectively loaded in the gels and analyzed by western blot to detect the quantitation of CHIP and RIPK3. Bars below the blots represent the mean ± SEM of four independent experiments. Significant differences ##p < 0.05 vs. Control, *p < 0.05 vs. ShNC, #p < 0.05 vs. ShNC, **p < 0.05 vs. ShCHIP. Related blots are shown in Supplementary Fig. S6. (C) Cell viability was tested after different treatment. Significant differences *p < 0.05 vs. ShNC.
