Figure 2 | Scientific Reports

Figure 2

From: Temporary sequestration of cholesterol and phosphatidylcholine within extracellular domains of ABCA1 during nascent HDL generation

Figure 2

Incubation-time dependence of trypsin-dependent PC and cholesterol release (A) and apoA-I–dependent PC and cholesterol efflux (B). BHK/Mock (○, dotted line) and BHK/ABCA1 (●, dotted line) cells were incubated with either 50 μg/mL trypsin (A) or 10 μg/mL apoA-I (B) for 0, 10, 30, or 60 min at 37 °C. After the incubation, amounts of PC and cholesterol in the medium were measured. ABCA1-specific release of PC and cholesterol (●, solid line) were calculated by subtracting the measured level of release from BHK/Mock cells from the measured level of release from BHK/ABCA1 cells. Experiments were performed in triplicate, and the average values are shown with S.D.

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