Table 2 Toxicity of vehicles in RPE65/rd12 mouse eye cup cultures.

From: Synthetic 9-cis-beta-carotene inhibits photoreceptor degeneration in cultures of eye cups from rpe65rd12 mouse model of retinoid cycle defect

Vehicle*

Concentration

Apoptosis score

Number of eye cups tested

None (growth media)

 

0

14

Phosphonium salt

0.01 mM

1

2

Phosphonium Salt

0.1 mM

1

2

Ethanol

5%

1

6

BHT

Volume equivalent to addition of 10 μM 9CBC

4

4

Miglyol 810

Volume equivalent to addition of 10 μM 9CBC

1

2

“antioxidant mix”*

Volume equivalent to addition of 10 μM 9CBC

1

6

Tween 40 and “antioxidant mix”*

Volume equivalent to addition of 10 μM 9CBC

4

3

Miglyol 810 and “antioxidant mix”*

Volume equivalent to addition of 1 μM 9CBC

1

3

Miglyol 810 and “antioxidant mix”*

Volume equivalent to addition of 10 μM 9CBC

3

3

  1. *“antioxidants mix” - a pellet content from the commercially available product CarotenALL, Mixed Carotenoid Complex (Jarrow Formulas) and 9CBC (10:5:1 w/w/w).
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