Figure 2
Starvation treatment induces increase of intracellular chloride concentration and suppression of CFTR. (A) Measurement of intracellular chloride concentration in nutrient-starved cells using the chloride-sensitive dye MQAE (blue). BF = bright field. Scale bars, 50 µm. Relative MQAE fluorescence intensity changes were quantified (bottom). All data are expressed as means ± SD. **P < 0.05. (B) Western blot analysis of CFTR expression in nutrient-starved cells. α-Tubulin served as a loading control. Quantitative data of optical band densitometry are shown. All data are expressed as means ± SD. **P < 0.05. (C) Western blot analysis of CFTR expression after termination of starvation. Means ± SD. **P < 0.05. (D) Immunofluorescence analysis of CFTR expression in cells nutrient-starved for 4 hours. DAPI stained the cell nuclei (blue). Green signals reflect the expression of CFTR. Scale bars, 50 µm.
