Figure 3
Inhibition of CFTR activity using GlyH-101 causes hyperpolarization and suppresses cell division. (A) Measurement of intracellular chloride concentration in GlyH-101 (10 nM)-treated cells using the chloride-sensitive dye MQAE (blue). BF = bright field. Scale bars, 50 µm. Relative MQAE fluorescence intensities were quantified (right). All data are expressed as means ± SD. (B) Membrane potential of cells treated with or without 10 nM GlyH-101, detected using DiBAC4(3) (green). BF = bright field. Scale bars, 50 µm. Relative DiBAC4(3) fluorescence intensity changes were quantified (right). All data are expressed as mean ± SD. (C) Western blot analysis of expression and phosphorylation of H3 in GlyH-101 (10 nM for 48 hours)-treated cells. Quantitative data of optical band densitometry are shown. Means ± SD. **P < 0.05. (D) BrdU incorporation assay and corresponding DAPI staining of GlyH-101–treated and DMSO-treated (control) cells. DAPI stained cell nuclei (blue). Red signals indicate BrdU staining. Scale bars, 50 µm. (E) Proliferation rates of GlyH-101 (10 nM)- and DMSO (control)-treated cells were assessed by the CCK-8 assay. All data are expressed as means ± SD.
