Figure 4
Starvation treatment induces a reversible increase in AMPK activity, and suppressing the increase in AMPK activity prevents hyperpolarization in nutrient-starved MKN45 cells. (A) Western blot analysis of expression and phosphorylation level of AMPK in nutrient-starved MKN45 cells. Quantitative data of optical band densitometry are shown. Means ± SD. **P < 0.05. (B) Western blot analysis of AMPK activity after termination of starvation. (C) Western blot analysis of expression and phosphorylation level of AMPK and H3 in nutrient-starved MKN45 cells, treated or not with compound C. Means ± SD. **P < 0.05. (D) Membrane potential of nutrient-starved cells, treated or not with 10 µM compound C for 4 hours, detected using DiBAC4(3) (green). BF = bright field. Scale bars, 50 µm. Relative DiBAC4(3) fluorescence intensity changes were quantified (bottom). All data are expressed as means ± SD. **P < 0.05.
