Figure 6
AICAR treatment inhibits cell division. (A) BrdU incorporation assay and corresponding DAPI staining of AICAR-treated and DMSO-treated (control) cells. Cell nuclei were stained with DAPI (blue). Red signals indicate BrdU staining. Scale bars, 50 µm. (B) Western blot analysis of expression and phosphorylation of H3 in AICAR-treated MKN45 cells. Quantitative data of optical band densitometry are shown. Means ± SD. **P < 0.05. (C) Proliferation rates of AICAR-treated cells were assessed by CCK-8 assay. All data are expressed as means ± SD. (D) Analysis of cell-cycle progression was performed by monitoring DNA content by flow cytometry after treatment with AICAR. Relative cell number in each phase was quantified (right). All data are expressed as means ± SD.
