Figure 7
Removal of AICAR induces recovery of membrane potential and cell-cycle progression. (A) MKN45 cells were pretreated with 1 mM AICAR for 18 hours, and the activity of AMPK was analyzed after removal of AICAR for 12 and 24 hours. Quantitative data of optical band densitometry are shown. Means ± SD. **P < 0.05. (B) Membrane potential of cells after removal of AICAR for 12 and 24 hours was detected using DiBAC4(3) (green). BF = bright field. Scale bars, 50 µm. Relative DiBAC4(3) fluorescence intensity changes were quantified (right). (C) Cell-cycle progression was analyzed by monitoring DNA content by flow cytometry after removal of AICAR. The relative cell number in each phase was quantified (right). All data are expressed as means ± SD.
