Figure 7

3B12A scFv-CMA reduces cytoplasmic TDP-43 aggregates in the murine cerebral cortex. (a) Myc-tagged 3B12A scFv-CMA colocalised with cytoplasmic TDP-43 aggregates in the cortical plate (CP, arrowheads). Scale bar = 50 μm. (b) Distribution of neurons expressing GFP-tagged WT and mutant TDP-43 in the E16.5 cortex. Electroporation was performed at E13.5, and brains were fixed at E16.5 followed by immunohistochemistry. Scale bar = 100 μm. (c–h) Quantification analysis of (b) TDP-43 aggregates were distinguished from non-aggregates by setting the fluorescence cut-off for the quantification using ImageJ software. Each data point was obtained by normalisation to mCherry. Differences were evaluated by unpaired t-test (mean ± SD, n = 7 for TDP-43^WT and n = 7 for TDP-43^{mNLS,C173S/C175S}; ****p < 0.001 vs empty vector). N.S. indicates not significant.