Figure 2
From: Visualizing multiple inter-organelle contact sites using the organelle-targeted split-GFP system
Split-GFP fragments on the ER and MOM label their contact sites. (A) Diagram of the split-GFP system for detecting inter-organelle interactions. (B) Yeast cells expressing Mmm1-GFP and mitochondria-targeted RFP (Su9-RFP) were imaged by confocal fluorescent microscopy. Maximum projection images reconstituted from the z-stacks were shown. Scale bar represents 5 µm. (C) Yeast cells expressing Tom71-GFP1-10 and Ifa38-mCherry-GFP11 were imaged by confocal fluorescent microscopy. Maximum projection images reconstituted from the z-stacks were shown. Scale bar represents 5 µm. (D,E) Yeast cells expressing split-GFP proteins, Ifa38-mCherry-GFP11 and Tom71-GFP1-10 (D) or Ifa38-GFP1-10 and Tom71-GFP11 as well as ER-targeted mCherry (E) were imaged by confocal fluorescent microscopy. Single focal plane images were shown. Dotted lines indicate plasma membranes. Scale bars represent 5 µm.
