Figure 6

Tranilast inhibits sNF96.2 cell proliferation and invasion in brain tissue. (a) sNF96.2 cells were injected into the brain of NOD/SCID mice. After 3 weeks, the mice were treated orally with tranilast (300 mg/kg) or vehicle once a day for 8 weeks. The brain was then removed from four representative mice, and formalin-fixed, paraffin-embedded sections of the xenograft tumours were subjected to immunohistochemical analysis with antibodies to vimentin. Scale bars, 100 µm. (b) sNF96.2-GFP cells were injected into the brain of NOD/SCID mice. After 6 weeks, the brain was removed and thin slices of brain tissue containing tumour cells were cultured in medium containing tranilast (750 µM) or DMSO vehicle for 0 or 5 days, at which times the same areas (Ct1–3 and Tra1–3) were examined by confocal fluorescence microscopy and photographed. Scale bars, 300 µm. Maximum tumour diameter and maximum length of the invasion path were determined from the images. Data are means ± s.d. (n = 3). *P < 0.05 versus corresponding tranilast value (Student’s unpaired t test).