Figure 1
From: Mitochondria-targeted Probes for Imaging Protein Sulfenylation
Characterization of DCP-NEt2C and DCP-Rho1 fluorescence and kinetic properties. (a) Chemical structure of DCP-Rho1. (b) DCP-NEt2C synthesis scheme and structure. (c) Excitation and emission spectra of DCP-NEt2C. Dotted line shows excitation spectrum taken with emission at λmax 499 nm; solid line line shows emission spectrum taken with excitation at λmax 421 nm. (d) Analysis of DCP-NEt2C (blue) and DCP-Rho1 (red) dependence of fluorescence signal on pH. (e) Deconvoluted mass spectra of oxidized C165A AhpC (black), and its 60 min reaction with dimedone (green), DCP-NEt2C (blue), and DCP-Rho1 (red). (f) SDS-PAGE fluorescence imaging analysis of oxidized C165A AhpC reaction with DCP-NEt2C and DCP-Rho1. (g) Kinetic profiles and reaction rates of oxidized C165A AhpC reaction with dimedone (black circle), DCP-NEt2C (open circle), and DCP-Rho1 (open square). The data were fit using single exponential kinetics and the second order rate constants were calculated by dividing the rate with the respective probe concentration.
