Figure 3
From: Structural and kinetic basis for the selectivity of aducanumab for aggregated forms of amyloid-β
Negative-stain electron microscopy of Aβ1-40 fibrils incubated with gold-conjugated anti-Aβ antibodies. Aβ1-40 fibrils were incubated with gold-conjugated chaducanumab (a), 3D6 (b), chgantenerumab (c) and m266 (d). 100 images were collected for each sample. A representative image for each data set is shown. Scale bars are 100 nm. (e) Labeling densities were calculated as the number of fibril-associated gold particles (distance of ≤10 nm to fibril) per 1 μm2 for each image, with mean ± SD for each group. The differences in the gold-labeled antibodies binding to Aβ fibrils were tested with a one-way ANOVA. P values of <0.0001 indicate significant differences between chaducanumab vs. 3D6, chgantenerumab and m266.
