Figure 4
From: Insights into aluminum-tolerance pathways in Stylosanthes as revealed by RNA-Seq analysis

Effect of Al3+ on the GTPase activity in the plasma membrane of R2 roots. (A) Immunoblot analyses for heterotrimeric G-proteins probed with antibody raised against the Arabidopsis Gα. (B) R2 seedlings were exposed to 0.5 mM CaCl2 solution containing 0 (−Al3+) or 50 μM AlCl3 (+Al3+) at pH 4.3 for 3 h. Vertical bars represent Mean ± SEM (n = 3). Pairwise Student’s t test was used to compare values to the control (−Al3+). **Significant at P < 0.01.