Figure 1
KLK12 exerts proteolytic activity against fibronectin isoforms. (A) Proteolysis of matrix proteins by KLK12, analyzed with SDS-PAGE and Coomassie blue staining (E:S molar ratio: 1:5, 4 h at 37 °C). (B) Characterization of FN proteolysis by KLK12, analyzed as in A. Left panel: Dose-dependent proteolysis of FN by KLK12 with an E:S molar ratio ranging from 1:5 to 1:1000; 4 h at 37 °C. Right panel: Time-dependent proteolysis of FN by KLK12, from 1 min to 5 h at 37 °C (E:S molar ratio: 1:50). (C) In control experiments, KLK12 was incubated with either E64 (100 µM), PMSF (0.5 mM) or EDTA (0.5 mM) and with FN. (D) Dose-dependent proteolysis of cellular FN isoforms (cFN and FN-EDB) by KLK12. E:S molar ratio: from 1:5 to 1:50; 4 h at 37 °C. The band corresponding to recombinant human KLK12 is indicated by an arrowhead when visible, and bands corresponding to proteolytic products are indicated by arrows.
