Figure 2
Relative abundance, alpha diversity, and dimensional reduction plot of CPV content microbiomes from NW vs O subjects. (A,B) Stacked column bar graphs depicting the different group average relative abundances and distribution of the most highly abundant resolved taxa at the (A) phylum and (B) family level for this study. (C) Cladograms derived from pairwise group LEfSe analysis of 16S sequences from CPV contents in NW vs O subjects. The cladograms show the taxonomic levels represented by rings with phyla at the innermost ring and genera at the outermost ring, and each circle is a member within that level. Taxa at each level are shaded (blue or red) according to the phenotype in which it is more abundant (P < 0.05; LDA score 2.0). (D) Bar graph analysis of the frequency of Deltaproteobacteria in both groups of subjects, significance is indicated. (E) Principal Component Orthogonal Analysis of the microbiota profile in both groups. The alpha cutoff is mentioned. (F) Plot of microbiome alpha diversity (Shannon index) for each CPV content sample at the phylum and family taxonomic levels. (G–K) Linear regression analyses between the frequency of the mentioned bacterial taxa and BMI. Significance, and linear regression coefficient are indicated on each panel. (L,M) Pathway component and structural components derived from pairwise group analysis of predicted metagenomics analysis (PICRUSt) of 16S sequences from CPV contents in NW vs O subjects. Major metabolic pathways are mentioned. (N,O) LDA Effect Size (LEfSe) cladograms of KEGG pathway contributions of predicted metagenomic data for CPV samples. Pairwise analysis was performed for NW vs O subjects. The cladograms show the KEGG pathway hierarchy represented by rings with the consolidated pathway modules (identified in the legend) at the outermost ring, and each circle is a member within that level. KEGG modules are shaded in color according to the group in which it is most abundant (P < 0.05; LDA score 2.0). The respective KEGG pathway cladogram legends are incorporated into the graphical representation (P,Q) of the relative predicted gene count for each differential feature.
