Figure 4

Relative abundance, alpha diversity, and dimensional reduction plot of CPV content microbiomes from OT and ONT subjects. (A,B) Stacked column bar graphs depicting the different group average relative abundances and distribution of the most highly abundant resolved taxa at the (A) phylum and (B) family level for this study. (C) Cladograms derived from pairwise group LEfSe analysis of 16S sequences from CPV contents in OT vs ONT subjects. Taxa at each level are shaded and colored according to the phenotype in which it is more abundant (P < 0.05; LDA score 2.0). (D) bar graph analysis of the frequency of Tepidimonas in both groups of subjects, significance is indicated. (E) Principal Component Orthogonal Analysis of the microbiota profile in both groups. The alpha cutoff is mentioned. (F) Plot of microbiome alpha diversity (Shannon index) for each CPV content sample at the phylum and family taxonomic levels. (G–J) linear regression analyses between the frequency of the mentioned bacterial taxa and BMI and (K–Q) with oro-sensory linoleic acid. Significance, and linear regression coefficient are indicated on each panel. (R,S) LDA Effect Size (LEfSe) cladograms of KEGG pathway component and structural components derived from pairwise group analysis of predicted metagenomics analysis (PICRUSt) of 16 S sequences from CPV contents in OT vs ONT subjects. Major metabolic pathways are mentioned. (T,U) of the relative predicted gene count for each differential feature.